Earticle

초록

영어

Epilepsy is a common neurological disorder characterized by seizure and loss of neuronal cells by abnormal rhythmic firing of neurons in the brain. In the present study, we investigated the effect of treadmill exer-cise on gamma-aminobutyric acid (GABA)ergic neuronal loss in relation with neuronal activation using pilocarpine-induced epileptic rats. The rats were divided into four groups: control group, control and treadmill exercise group, pilocarpine-induced epilepsy group, and pilocarpine-in-duced epilepsy and treadmill exercise group. Epilepsy was induced by intraperitoneal injection of 320 mg/kg pilocarpine hydrochloride. The rats in the exercise groups were forced to run on a motorized treadmill for 30 min once a day for 2 weeks. In the present results, neuronal loss in the hippocampal CA1 region was increased after pilocarpine-in-duced seizure. Treadmill exercise inhibited hippocampal neuronal loss in the epileptic rats. Glutamic acid decarboxylase (GAD67) expression in the hippocampal CA1 region was reduced by pilocarpine-induced seizure. Treadmill exercise increased GAD67 expression in the epileptic rats. c-Fos expression in the hippocampal CA1 region was increased in response to epileptic seizure. Treadmill exercise inhibited c-Fos expres-sion in the epileptic rats. Epileptic seizure increased brain-derived neu-rotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) expres-sions in the hippocampus. Treadmill exercise suppressed BDNF and TrkB expressions in the epileptic rats. In the present study, treadmill ex-ercise prevented GABAergic neuronal loss and inhibited neuronal acti-vation in the hippocampal CA1 region through the down-regulation of BDNF-TrkB signaling pathway.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Experimental animals
  Pilocarpine-induced epilepsy model
  Treadmill exercise protocol
  Tissue preparation
  Nissl staining
  GAD67 immunohistochemistry
  c-Fos immunohistochemistry
  Western blot analysis for BDNF andTrkB
  Statistical analysis
 RESULTS
  Effect of treadmill exercise on the number of pyramidal neurons in the hippocampal CA1 region
  Effect of treadmill exercise on the number of GAD67-positive cells in the hippocampal CA1 region
  Effect of treadmill exercise on the number of c-Fos-positivecells in the hippocampal CA1 region
  Effect of treadmill exercise on the expressions of BDNFand trkB in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Baek-Vin Lim [ Department of Exercise Prescription, Division of Leisure & Sports Science. Dongseo University, Busan, Korea ]
• Mal-Soon Shin [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jae-Min Lee [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Jin-Hee Seo [ Division of Sports Science, Baekseok University, Cheonan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613