Earticle

초록

영어

Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on 1H-NMR, 13C-NMR spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration (IC50) of 8.2 μg/mL and 3.1 μg/mL, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

한국어

많은 식물들은 부작용이 적고, 가격이 저렴하며, 다양한 웰니스 융복합 화합물들을 함유하고 있기 때문에 다양한 제재에 이용되고 있다. 이 연구에서 뉴질랜드 식물인 Quintinia acutifolia (Q. acutifolia)로부터 쥐 백혈병 세 포(P388 murine lymphocytic leukemia cells)의 성장을 저해하는 활성을 MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay에 의해 평가하였다. P388 murine lymphocytic leukemia 세포의 성장을 저해하 는 2,3,2'',3''-tetrahydroochanaflavone (1)과 2'',3''-dihydroochana-flavone (3)을 1D/2D-NMR와 다른 분광학적 분석법에 의해 분리하였고, 구조를 규명하였다. 이 두 화합물은 두 개의 플라보노이드 기본구조를 갖는 바이플라보노이드 (biflavonoid)로써 2,3,2'',3''-Tetrahydroochnaflavone (1)과 2'',3''-dihydroochana-flavone (3) 화합물은 P388 murine lymphocytic leukemia세포에 대해 50%의 성장저해를 나타내는 농도가 각각 8.2 μg/mL와 3.1 μg/mL로 나타났다. 특 히 2'',3''-dihydroochana-flavone (3) 화합물은 2,3,2'',3''-tetrahydroochanaflavone (1)의 B 링(ring)에 쌍으로 결합되지 않은 플라본 구조 (unconjugated flavonone system)를 갖는 것으로 나타났다. 그럼으로 두 화합물은 향후 항암 치료제 개발에 이용될 수 있으며, 더 많은 연구가 요구된다.

목차

Abstract
 요약
 1. Introduction
 2. Materials and Methods
  2.1 General experimental procedures
  2.2 Plant materials
  2.3 Extraction and isolation
  2.4 HPLC Method for analysis of 2,3,2'',3'’-tetrahydroochanaflavone (1)
  2.5 Screening for cytotoxic activity
 3. Results and Discussion
 4. Conclusion
 REFERENCES

키워드

웰니스 융복합 항암 생리활성 뉴질랜드 식물 Wellness-fusion-complex Anti-cancer Funtional materials Biflavonoid New Zealand plant P388murine lymphocytic leukemia cells Biflavonoid P388 murine lymphocytic leukemia cells

저자

• Jae-Sook Lee [ 이재숙 | Dep. of Beauty Science, Gwangju Women's University ]
• Hwa-Jung Choi [ 최화정 | Dep. of Beauty Science, Gwangju Women's University ]
• Myung-Ju Kim [ 김명주 | Dep. of Hair stylist, Skin, Gwangju Health University ]
• Jang-Soon Park [ 박장순 | Dep. of Beauty Art, Songwon University ] Corresponding Author

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