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Expression of Sirt1, Sirt2, Sirt5, and Sirt6 in the Mouse Testis

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 39 No 2 (2015.05)바로가기
  • 페이지
    pp.43-47
  • 저자
    Byeong Seong Ki, Miree Park, Yunmi Woo, Woo Sik Lee, Jung Jae Ko, Youngsok Choi
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A248362

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초록

영어
Sirtuin proteins are evolutionary conserved Sir2-related NAD+-dependent deacetylases and regulate many of cellular processes such as metabolism, inflammation, transcription, and aging. Sirtuin contains activity of either ADP-ribosyltransferase or deacetyltranfease and their activity is dependent on the localization in cells. However, the expression pattern of Sirtuins has not been well studied. To examine the expression levels of Sirtuins, RT-PCR was performed using total RNAs from various tissues including liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Sirtuins were highly expressed in most of tissues including the testis. Immunostaining assay showed that Sirt1 and Sirt6 were mainly located in the nucleus of germ cells, spermatocytes, and spermatids in the seminiferous tubules, whereas Sirt2 and Sirt5 were exclusively present in the cytoplasm of germ cells and spermatocytes. Our results indicate that Sirtuins may function as regulators of spermatogenesis and their activities might be dependent on their location in the seminiferous tubules.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  RT-PCR Analysis
  Immunostaining
 RESULTS
 DISCUSSION
 ACKNOWLEDGMENTS
 REFERENCES

키워드

Sirtuin Sirt1 Sirt2 Sirt5 Sirt6 Mouse Testis

저자

  • Byeong Seong Ki [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ]
  • Miree Park [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ]
  • Yunmi Woo [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ]
  • Woo Sik Lee [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ]
  • Jung Jae Ko [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ]
  • Youngsok Choi [ Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 39 No 2

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