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Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 39 No 2 (2015.05)바로가기
  • 페이지
    pp.29-36
  • 저자
    Tao Lin, Jae Eun Lee, Hyun Young Shin,, Reza K. Oqani, Dong Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A248360

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초록

영어
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.

목차

ABSTRACT
 INTRODUCTION
 FOLLICLE DIAMETER
 COC MORPHOLOGY
 OOCYTE RECOVERY METHOD
 IVM OF OOCYTES
 DENUDING PROCEDURES
 MORPHOLOGY OF THE FIRST POLAR BODY
 EMBRYO CULTURE MEDIUM AND CONDITIONS
 RELATIONSHIP BETWEEN EMBRYO QUALITY AND CULTURE TIME
 IN VITRO FERTILIZATION
 PARTHENOGENETIC ACTIVATION
 SOMATIC CELL NUCLEAR TRANSFER
 IN SUMMARY
 REFERENCES

저자

  • Tao Lin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
  • Jae Eun Lee [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
  • Hyun Young Shin, [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
  • Reza K. Oqani [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
  • Dong Il Jin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 39 No 2

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