Tao Lin, Jae Eun Lee, Hyun Young Shin,, Reza K. Oqani, Dong Il Jin
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https://www.earticle.net/Article/A248360
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영어
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
목차
ABSTRACT INTRODUCTION FOLLICLE DIAMETER COC MORPHOLOGY OOCYTE RECOVERY METHOD IVM OF OOCYTES DENUDING PROCEDURES MORPHOLOGY OF THE FIRST POLAR BODY EMBRYO CULTURE MEDIUM AND CONDITIONS RELATIONSHIP BETWEEN EMBRYO QUALITY AND CULTURE TIME IN VITRO FERTILIZATION PARTHENOGENETIC ACTIVATION SOMATIC CELL NUCLEAR TRANSFER IN SUMMARY REFERENCES
Tao Lin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
Jae Eun Lee [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
Hyun Young Shin, [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
Reza K. Oqani [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
Dong Il Jin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Republic of Korea ]
Corresponding author