Earticle

초록

영어

Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cultured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non- proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn’t stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animal
  Isolation of SSCs from Porcine Testes
  Adherent Culture of Porcine SSCs
  Suspension Culture of Porcine SSCs
  AP Staining
  Real-Time Polymerase Chain Reaction (PCR)
  Statistical Analysis
 RESULTS
  Effects of Suspension Culture on Self-Renewal of SSCs Derived from Porcine Neonatal Testis
  Effects of Long-Term Suspension Culture on Self-Renewal of SSCs Derived from Porcine Neonatal Testis
 DISCUSSION
 REFERENCES

키워드

저자

• Min Hee Park [ Department of Animal Life Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Ji Eun Park [ Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Min Seong Kim [ Department of Animal Life Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Kwon Young Lee [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Jung Im Yun [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Jung Hoon Choi [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Eunsong Lee [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea ]
• Seung Tae Lee [ Department of Animal Life Science, Kangwon National University, Chuncheon 200-701, Korea, Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636