Earticle

초록

영어

MicroRNAs (miRNAs) are approximately 22 nucleotides of small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The miRNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, metabolism, imprinting, and differentiation. Recently, a paper has shown that expression of the miRNA-302/367 cluster expressed abundantly in mouse and human embryonic stem cells (ESCs) can directly reprogram mouse and human somatic cells to induced pluripotent stem cells (iPSCs) efficiently in the absence of any of the four factors, Oct4, Sox2, c-Myc, and Klf4. To apply this efficient method to porcine, we analyzed porcine genomic sequence containing predicted porcine miRNA-302/367 cluster through ENSEMBL database, generated a non-replicative episomal vector system including miRNA-302/367 cluster originated from porcine embryonic fibroblasts (PEF), and tried to make porcine iPSCs by transfection of the miRNA-302/367 cluster. Colonies expressing EGFP and forming compact shape were found, but they were not established as iPSC lines. Our data in this study show that pig miRNA-302/367 cluster could not satisfy requirement of PEF reprogramming conditions for pluripotency. To make pig iPSC lines by miRNA, further studies on the role of miRNAs in pluripotency and new trials of transfection with conventional reprogramming factors are needed.

목차

ABSTRACT
 INTRODUCTION
 MATERIAL AND METHODS
  Porcine miRNA-302/367 Cluster Search
  MicroRNA Expression Vector Construction
  Cell Culture and Transfection
 RESULTS
  Identification of Porcine miRNA-302/367 Cluster
  Somatic Cell Reprogramming by Porcine miRNA-302/367 Cluster Transfection
 DISCUSSION
 REFERENCES

키워드

저자

• Dong-Chan Son [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture Life Sciences, Seoul National University, Seoul 151-921, Korea ]
• Jae Yeon Hwang [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture Life Sciences, Seoul National University, Seoul 151-921, Korea ]
• Chang-Kyu Lee [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture Life Sciences, Seoul National University, Seoul 151-921, Korea ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636