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Genetic Diversity of Nigerian Date Palm (Phoenix dactylifera) Germplasm based on Microsatellite Markers

첫 페이지 보기
  • 발행기관
    보안공학연구지원센터(IJBSBT) 바로가기
  • 간행물
    International Journal of Bio-Science and Bio-Technology SCOPUS 바로가기
  • 통권
    Vol.7 No.1 (2015.02)바로가기
  • 페이지
    pp.121-132
  • 저자
    A.O. Yusuf, A. Culham, W. Aljuhani, C.D. Ataga, A. M. Hamza, J.O. Odewale, L. O. Enaberue
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A241808

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원문정보

초록

영어
Characterization of date palm cultivars is a complex task using morphological traits alone since morphological markers are dependent on plant developmental stage and influenced by the environment. However, DNA fingerprinting can complement and enhance the discriminatory power of morphological traits. The study was conducted to investigate genetic diversity amongst fourteen cultivars of date palm (Phoenix dactylifera L.) from Nigeria and Saudi Arabia using microsatellite markers. The aim was to determine the genetic and geographical patterns of Nigeria and Saudi Arabia date palms. Molecular study conducted using six microsatellite markers employed on fourteen cultivars, ten from Nigeria and four from Saudi Arabia, revealed 83.3% polymorphism which indicated high genetic diversity among the cultivars studied. The amplified products ranged in size from 127 to 304 bp. A total of 42 alleles with an average of seven alleles per locus were scored. Two of the markers, MpdCIR025 and MpdCIR050, distinctively characterized six cultivars. This study indicated that variation observed among the cultivars followed a geographical pattern. However, this study was not able to show any alleles that might be linked to gender in date cultivars. Inclusion of more molecular markers in such a study might provide more accurate differentiation and possibly gender discrimination in date palm.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Plant Materials
  2.2. Location
  2.3. DNA Extraction
  2.4. Polymerase Chain Reaction (PCR) Analysis
  2.5. Data Analysis
 3. Results and Discussions
  3.1. DNA Extraction and PCR
  3.2. SSR Amplification
  3.3. Level of Polymorphism
  3.4. Genetic Diversity
 4. Conclusion
 Acknowledgements
 References

키워드

Characterization Genetic diversity Date palm Microsatellite markers Nigeria

저자

  • A.O. Yusuf [ Plant Breeding Division, Nigerian Institute for Oil Palm Research (NIFOR), P.M.B 1030, Benin City, 300001, Nigeria, Centre of Plant systematics and Diversity, School of Biological Science, Harbourne Building, University of Reading, RG6 6AS, Reading, United Kingdom ]
  • A. Culham [ Centre of Plant systematics and Diversity, School of Biological Science, Harbourne Building, University of Reading, RG6 6AS, Reading, United Kingdom ]
  • W. Aljuhani [ Centre of Plant systematics and Diversity, School of Biological Science, Harbourne Building, University of Reading, RG6 6AS, Reading, United Kingdom ]
  • C.D. Ataga [ Plant Breeding Division, Nigerian Institute for Oil Palm Research (NIFOR), P.M.B 1030, Benin City, 300001, Nigeria ]
  • A. M. Hamza [ Nigerian Institute for Oil palm Research Outreach Centre, Wamba, Nigeria ]
  • J.O. Odewale [ Plant Breeding Division, Nigerian Institute for Oil Palm Research (NIFOR), P.M.B 1030, Benin City, 300001, Nigeria ]
  • L. O. Enaberue [ Plant Breeding Division, Nigerian Institute for Oil Palm Research (NIFOR), P.M.B 1030, Benin City, 300001, Nigeria ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    보안공학연구지원센터(IJBSBT) [Science & Engineering Research Support Center, Republic of Korea(IJBSBT)]
  • 설립연도
    2006
  • 분야
    공학>컴퓨터학
  • 소개
    1. 보안공학에 대한 각종 조사 및 연구 2. 보안공학에 대한 응용기술 연구 및 발표 3. 보안공학에 관한 각종 학술 발표회 및 전시회 개최 4. 보안공학 기술의 상호 협조 및 정보교환 5. 보안공학에 관한 표준화 사업 및 규격의 제정 6. 보안공학에 관한 산학연 협동의 증진 7. 국제적 학술 교류 및 기술 협력 8. 보안공학에 관한 논문지 발간 9. 기타 본 회 목적 달성에 필요한 사업

간행물

  • 간행물명
    International Journal of Bio-Science and Bio-Technology
  • 간기
    격월간
  • pISSN
    2233-7849
  • 수록기간
    2009~2016
  • 등재여부
    SCOPUS
  • 십진분류
    KDC 505 DDC 605

이 권호 내 다른 논문 / International Journal of Bio-Science and Bio-Technology Vol.7 No.1

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