Earticle

초록

영어

We analyzed the differential effects of histopathology, apoptosis and expression of radiation response genes after chronic low dose rate (LDR) and acute high dose rate (HDR) radiation exposure in spleen, lung and liver of rats. Female 6-week-old Sprague-Dawley rats were used. For chronic low-dose whole body irradiation, rats were maintained for 14 days in a 60Co gamma ray irradiated room and received a cumulative dose of 2 Gy or 5 Gy. Rats in the acute whole body exposure group were exposed to an equal dose of radiation delivered as a single pulse (137Cs-gamma). At 24 hours after exposure, spleen, lung and liver tissues were extracted for histopathologic examination, western blotting and RT-PCR analysis. 1. The spleen showed the most dramatic differential response to acute and chronic exposure, with the induction of substantial tissue damage by HDR but not by LDR radiation. Effects of LDR radiation on the lung were only apparent at the higher dose (5 Gy), but not at lower dose (2 Gy). In the liver, HDR and LDR exposure induced a similar damage response at both doses. RT-PCR analysis identified cyclin G1 as a LDR-responsive gene in the spleen of rats exposed to 2 Gy and 5 Gy gamma radiation and in the lung of animals irradiated with 5 Gy. 2. The effects of LDR radiation differed among lung, liver, and spleen tissues. The spleen showed the greatest differential effect between HDR and LDR. The response to LDR radiation may involve expression of cyclin G1.

목차

Abstract
 1. INTRODUCTION
 2. MATERIALS AND METHODS
 3. RESULTS
  3.1 Acute HDR radiation induces more serious organ damage than chronic LDR radiation, particularly in the spleen
  3.2 Cleavage of caspase 3 by HDR or LDR radiation
  3.3 Differential gene expression patterns in the spleen according to LDR or HDR exposure
  3.4 Differential gene expression patterns in lung tissueaccording to LDR or HDR exposure
  3.5 Differential gene expression patterns in liver tissueaccording to LDR or HDR exposure
 4. DISCUSSION
 Acknowledgements:
 Declaration Of Interest
 REFERENCES

키워드

저자

• Hee Jin [ Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul Korea, These authors contributed equally to the study. ]
• Yeung Bae Jin [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea, These authors contributed equally to the study. ]
• Ju-Woon Lee [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea ]
• Jae-Kyung Kim [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea ]
• Yun-Sil Lee [ Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  방사선방어학회지 [Journal of Radiation Protection and Research]

• 간기

  계간

• pISSN

  2508-1888

• 수록기간

  1976~2026

• 등재여부

  KCI 등재,SCOPUS

• 십진분류

  KDC 559 DDC 629