The aim of this study was to assess the in vitro potential of methanolic seed extract of Abelmoschus esculentus as a natural antioxidant. The DPPH activity of the Ethyl acetate soluble fraction (10, 20, 40, 80, and 160 μg/mL) was increased in a dose dependent manner, which was found in the range of 18.97-90.47% as compared to ascorbic acid 26.44-93.71%. The IC50 values of Ethyl acetate soluble fraction (EAES) and ascorbicacid in DPPH radical scavenging assay were obtained to be 28.12 and 18.43 μg/mL, respectively. Measurement of polyphenol content of the EAES of A. esculentus seed was achieved using Folin-Ciocalteau reagent containing 53.80 mg/g of total phenolic content, which was found signicantly higher when compared to reference standard gallic acid. Similarly total flavonoids and proabthocyanidis of EAES and chloroform soluble fraction (CAES) were found significantly 147 mg/g and 14.24 mg/g respectively when both compared to reference standard quercetin. EAES exhibited high significant lipid peroxidation inhibition effects in a dose- dependent manner, with IC50 values of 38.08 μg/mL, whereas, standard quercetin, with IC50 value of 36.67 μg/mL. All extract/ fractions showed dose dependent reducing power ability and these differences were statistically significant (p<0.001). The results obtained in this study clearly indicate that A. esculentus seed has a signicant potential to use as a natural antioxidant agent.
목차
Abstract 1. Introduction 2. Experimental Section 2.1 Plant Materials and Extraction 2.2 Drugs and Chemicals 2.3 In vitro Antioxidant Activity 2.4 Free Radical Scavenging Activity Measured by 1, 1-Dipheny1-2-picryl-hydrazyl(DPPH) 2.5. Reducing Power Activity 2.6. Lipid Peroxidation Inhibition Assay 2.7. Statistical Analysis 3. Results and Discussion 3.1. Total Phenol, Flavonoid and Proanthocyanidin Content 3.2. Total Antioxidant Capacity 3.3. DPPH Radical Scavenging Activity 3.4. Lipid Peroxidation Inhibition Activity 3.5. Reducing Power Ability 4. Conclusions Abbreviation
키워드
A. Esculentus SeedAntioxidantDPPHLipid PeroxidationReducing Power
저자
Md. Saifur Rahman [ Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, South Korea ]
Jin Cheol Yoo [ Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, South Korea ]
Corresponding author
조선대학교 기초과학연구원 [The Natural Science Research Institute of Chosun]
설립연도
2008
분야
자연과학>자연과학일반
소개
본 연구원은 기초과학을 진흥하기 위한 연구·교육 및 그 보급을 목적으로 한다. 이 목적을 달성하기 위하여 다음 각 호의 사업을 수행한다.
1. 기초과학 제 분야에 관한 조사와 연구
2. 기초과학에 관한 학술행사(학술대회, 학술세미나, 심포지엄, 초청강연회 등) 개최
3. 학문후속세대 및 일반인을 위한 기초과학 교육
4. 기관지『조선자연과학논문지』 발간
5. 『자연과학연구총서』, 『자연과학번역총서』 등 단행본 발간
6. 기타 본 연구원의 목적과 관련된 사업
간행물
간행물명
통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]