Earticle

초록

영어

Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a 15 μM of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a 100 μM concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Sang-Hoon Park [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea ]
• Mi-Ran Lee [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea ]
• Tae-Suk Kim [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea,Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea ]
• Sang-Ki Baek [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea ]
• Sang-Jin Jin [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea ]
• Jin-Wook Kim [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea,Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea ]
• Sang-Gon Jeon [ Department of Food and Resource Economics, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea, Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea ]
• Ho-Baek Yoon [ Dairy Science Division, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Republic of Korea ]
• Joon-Hee Lee [ Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea,Institute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea ] Corresponding author

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간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636