Sung-I Woo, Jae-Gu Han, Pyung-Gyun Shin, Youn-Lee Oh, Song-Hee Lee, Kyung-Soo Kim, Sung-Hwan Jo, Won-Sik Kong
언어
영어(ENG)
URL
https://www.earticle.net/Article/A233923
원문정보
초록
영어
A total of 25 strains of Flammulina velutipes were analyzed to identify the genomic regions responsible for producing white-fruiting body. NGS data was yielded by using Illumina Hiseq platform. Short reads were filtered by quality score and read length were mapped on the reference genome (KACC42780). Between the white- and brown fruitbody forming strains, we found 9376 SNPs, of which 8178 were non-polymorphic and 1198 were polymorphic. There is a high possibility that SNPs can be detected among the white strains as homozygous because white phenotype is recessive in F. velutipes. Thus, we constructed SNP matrix within 8 white strains. SNPs discovered between mono3 and mono19, the parental monokaryotic strains of 4120 strain (white), were excluded from the candidate. If the genotypes of SNPs detected between white and brown strains were identical with those in mono3 and mono19 strains, they were included in candidate as a priority. As a result, if more than 5 candidates SNPs were localized in single gene, we regarded as they are possibly related to the white color. In F. velutipes genome, chr08: 950kb-2650kb, chr09: 500kb-1400kb, chr09: 2800kb-4350kb, and chr11: 2450kb-3500kb regions were identified to be associated with white fruitbody forming.
저자
Sung-I Woo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]
Jae-Gu Han [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]
Pyung-Gyun Shin [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]
Youn-Lee Oh [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]
Song-Hee Lee [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]
Kyung-Soo Kim [ Department of Applied Biology Graduate School, Kangwon National University , Chuncheon 200-701, Korea ]
Sung-Hwan Jo [ SEEDERS, Daeduk lndustry Academic Corperation Building, Gwanpyeong-dong Yuseong-gu, Daejeon, Republic of Korea ]
Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration Chungbuk Eumsung 369-873, Korea ]