Earticle

초록

영어

Interleukin–17E (also known as Interleukin–25) is the distinct member of Interleukin–17 cytokine family, induces the expression of IL–4, IL–5 and IL–13. Economical production of interleukin – 17E has lot of importance in the current day research in several clinical applications. The objective of the study was to optimize the physico–chemical parameters i.e., dissolved oxygen (DO) and nutritional factors i.e., carbon, nitrogen and phosphate sources on production of Interleukin–17E using industrially important salt inducible Escherichia coli GJ1158. The expression levels were not increased beyond 30 % DO in batch fermentation, but expression levels were increased beyond 30 % DO in fed-batch fermentation. The threshold level of dissolved oxygen ranges was 50 % in respect to the IL–17E production. Pulses of nutritional factors i.e., glucose, yeast extract and K2HPO4 enhanced the expression levels in fed batch fermentation at 40 % and 50 % dissolved oxygen ranges. When OD600 of the culture reaches to 74 in fed batch fermentation, culture was induced with 100 mM sterile NaCl and further incubated for next 15 hr. Purification was carried out using Ni – NTA spin column. A final concentration of 98 mg/L of purified IL–17E was obtained using cost effective medium viz., modified M9ON medium. The IL–17E thus produced is tested for its activity. In this study, fed-batch fermentation emphasizes the highest concentration of codon optimized recombinant human interleukin–17E using salt inducible expression host till to date, which manages to satisfy the industrial and clinical requirements.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Bacterial Strains and Culture Conditions
  2.2. Preparation of Inoculum
  2.3. Shake Flask Fermentation
  2.4. Fermentation
  2.5. Refolding and Purification of rhIL–17E
  2.6. Biological Activity of rhIL-25
 3. Results and Discussion
  3.1. Shake Flask Cultivation
  3.2. Batch Fermentation
  3.3. Fed Batch Fermentation
  3.4. Purification and Activity of rhIL–17E
 4. Conclusion
 Acknowledgements
 References

키워드

저자

• Jaya Lakshmi G [ Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India ]
• Seetha Ram Kotra [ Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India ]
• PVD Soujanya Kumari [ Department of Microbiology, M.V.R. PG College, Andhra University, Visakapatnam- 530026, India ]
• P Balakota Reddy [ Department of Research and Development, Bioviz Technologies Pvt Ltd, Hyderabad- 500029, India ]
• JB Peravali [ Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India ]
• KRS Sambasiva Rao [ Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Advanced Science and Technology

• 간기

  월간

• pISSN

  2005-4238

• 수록기간

  2008~2016

• 십진분류

  KDC 505 DDC 605