Earticle

초록

영어

Schizophrenia is a severe mental disorder characterized by abnormal mental functioning and disruptive behaviors. Abnormal expression of N-methyl-D-aspartate (NMDA) receptor, one of the glutamate receptor subtypes, has also been suggested to contribute to the symptoms of schizophrenia. The effect of treadmill exercise on schizophrenia-in-duced apoptosis in relation with NMDA receptor has not been evaluat-ed. In the present study, we investigated the effect of treadmill exercise on neuronal apoptosis in the hippocampus using MK-801-induced schizophrenic mice. MK -801 was intraperitoneally injected once a day for 2 weeks. The mice in the exercise groups were forced to run on a treadmill exercise for 60 min, once a day for 2 weeks. In the present re-sults, repeated injection of the NMDA receptor antagonist MK-801 re-duced expression of NMDA receptor in hippocampal CA2-3 regions. MK-801 injection increased casapse-3 expression and enhanced cyto-chrome c release in the hippocampus. The ratio of Bax to Bcl-2 was high-er in the MK-801- induced schizophrenia mice than the normal mice. In contrast, treadmill exercise enhanced NMDA receptor expression, suppressed caspae-3 activation and cytochrome c release, and inhibited the ratio of Bax to Bcl-2. Based on present finding, we concluded that NMDA receptor hypofunctioning induced neuronal apoptosis in MK-801-induced schizophrenic mice. Treadmill exercise suppressed neuro-nal apoptosis through enhancing NMDA receptor expression in schizo-phrenic mice.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animal and experimental design
  Preparation of MK-801-induced schizophrenia model in mice
  Exercise protocol
  Tissue preparation
  Immunohistochemistry for NMDA receptor and caspase-3
  Western blot for Bax, Bcl-2, and cytochrome c
  Data analysis
 RESULTS
  Effect of treadmill exercise on the number of NMDA receptor in the hippocampal CA2-3
  Effect of treadmill exercise on the number of caspase-3-positive cells in hippocampal dentate gyrus
  Effect of treadmill exercise on Bax and Bcl-2 expressions in the hippocampus
  Effect of treadmill exercise on cytochrome c expression in the hippocampus
 DISCUSSION
 CONFLICT OF INTEREST
 ACKNOWLEDGMENTS
 REFERENCES

키워드

저자

• Jin Woo Chung [ Department of Urology, Gachon University Gil Medical Center, Gachon University, Incheon, Korea ]
• Jin-Hee Seo [ Division of Sports Science, Baekseok University, Cheonan, Korea ]
• Sang-Bin Baek [ Department of Psychiatry, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613