Earticle

초록

영어

This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods. Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.

목차

Abstract
 Introduction
 Materials and Methods
  Plant materials and DNA extraction
  PCR amplification
  Sequencing of PCR products
 Results and Discussion
 Acknowledgements
 References

키워드

저자

• Yurry Um [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-871, Republic of Korea ]
• Won-Kyu Park [ Departments of Wood & Paper Science, ]
• Nam-Su Jo [ Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Republic of Korea ]
• Yi Lee [ Plant Science & Technology, Chungbuk National University, Cheongju 361-763, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634