The efficacy of forensic genotyping as an investigative tool has led to the continual expansion of national DNA databases as genotype profiles are added at an ever-increasing rate. To decrease the risk of false matches in the growing DNA databases, ENFSI and EDNAP recommended an extension of the European Standard Set (ESS) of loci. Following these recommendations, STR multiplexes have been developed by several commercial companies with the extended ESS of loci. AmplSTRⓇ NGM SElectTM PCR Amplification kit (Applied Biosystems) is the next generation kit contains amplify 10 loci plus the 5 new markers (D10S1248, D1S1656, D22S1045, D2S441, D12S391) recommended by ENFSI and EDNAP plus the highly discriminating SE33. With the aim of increasing STR markers of the missing children DNA databases in Korea we introduced new multiplex STR kit, AmplSTRⓇ NGM SElectTM kit (Applied Biosystems). In this study, we present the results of forensic validation studies with the NGM SElectTM kit including following aspects: reproducibility, sensitivity, performance on forensic samples, mixture sample analysis and kinship analysis. The NGM SElectTM kit showed comparable performances to the previously developed STR kits. NGM SElectTM kit showed improved performances, especially in regard to its sensitivity. Kinship analysis and forensic casework sample analysis result showed the NGM SElectTM kit are very informative for forensic purposes and the 6 additional loci provides a good tool for deficiency paternity cases.
목차
Abstract I. 서론 II. 실험 방법 1. 시료 2. DNA분리 3. PCR 증폭 4. 전기영동 III. 실험결과 1. 반복성 시험 (Reproducibility studies) 2. 민감도 시험 (Sensitivity and stochastic studies) 3. 혼합반 시료의 분석능력 시험 (Mixture studies) 4. 현장시료(뼈 시료)에 대한 분석능력 시험 (forensic sample analysis) 5. 친자관계 확인시험 (Kinship tests and deficiency paternity cases) IV. 고찰 V. 감사의 글 VI. 참고문헌
키워드
NGM SElectTM kitDNA databasesEuropean Standard Setkinship analysis.
법과학 분야는 사회정의 구현에 있어 크나큰 가치가 있음에도 불구하고 우리나라에서는 이 분야에 대한 인식이 미흡하여 선진 외국에 비해 침체되어 있는 실정이다. 이에 우리나라에서도 법과학 분야와 관련 있는 학계, 연구기관, 수사기관 등 유관 단체들로 구성된 한국 법과학회를 창립하여 이 분야를 활성화 시켜 과학수사를 한층 더 발전시키기 위함을 목적으로 한다.