Earticle

초록

영어

Objective: To analyze the potential beneficial effects and mechanisms of action of resveratrol on the maturation of bovine oocytes that were incubated in different concentrations of resveratrol (0.1, 1.0 or 10μM) as germinal vesicle (GV) oocytes. Design: In vitro prospective study. Setting: University research laboratory Patient(s): Animal models for human studies. Intervention(s): In vitro culture in the presence of various concentrations of the antioxidant resveratrol. Main Outcome Measures: The parameters of hormone levels, oocyte nuclear maturation, cumulus expansion, levels of intracellular glutathione and reactive oxygen species, embryonic cleavage, blastocyst formation, gene expression associated with mature bovine oocytes and cumulus cells and the level of sirtuin 1 gene expression were detected. Results: Resveratrol significantly increased progesterone secretion and decreased estradiol- 17β secretion by cumulus cells. The elevated levels of progesterone activated the Mos/MEK/p42 MAPK cascade in the oocytes. At a concentration of 1.0 μM, resveratrol significantly improved cumulus expansion, polar body formation, the (hatched)blastocyst rate and the mean number of cells/blastocyst. Meanwhile, resveratrol significantly reduced the level of ROS, increased the level of GSH. For the first time, the expression of the sirtuin 1 gene was identified in granulosa cells, cumulus cells, oocytes and blastocysts. Further studies revealed that resveratrol promoted sirtuin 1 gene expression.Conclusion(s): Resveratrol promoted bovine oocyte maturation and subsequent post- IVF embryonic development by inducing progesterone secretion and antioxidant effect, probably in a manner dependent on Sirtuin 1.

키워드

저자

• Feng Wang [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• XiuZhi Tian [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• Lu Zhang [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• ChangJiu He [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• PengYun Ji [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• Yu Li [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]
• DunXian Tan [ Department of Cellular & Structural Biology, The UT Health Science Center ]
• GuoShi Liu [ State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology China Agricultural University ]

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  한국동물번식학회 한중일 심포지엄

• 간기

  연간

• 수록기간

  2014~2014

• 십진분류

  KDC 527 DDC 636