Earticle

현재 위치 Home

The Molecular Mechanisms Underlying Active Immunization against GnRH (Immuno-Castration): using Rat as a Model

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    한국동물번식학회 한중일 심포지엄 바로가기
  • 통권
    The 3rd Korea-China-Japan Joint Symposium on Animal Reproduction (2014.06)바로가기
  • 페이지
    pp.4-4
  • 저자
    Xing-fa Han, Xian-yin Zeng
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A227387

원문정보

초록

영어
The present study was aimed to investigate the molecular mechanisms of active immunization against GnRH by focusing on effects of active immunization against GnRH on hypothalamic-pituitary-testicular axis. Adult male rats (n=36) were randomly and equally allocated into three groups: control (no treatment), surgically castrated, or immunized against 50 ug D-Lys6-GnRH-tandem-dimer peptide conjugated to ovalbumin in Specol adjuvant at 12 weeks of age (with a booster 8 weeks later). Blood samples (for antibody titers and hormone concentrations) were collected at 2-week intervals until rats were killed at 20 weeks of age. At decapitation, testes weight /volume and pituitary weight were recorded. Hypothalamus, pituitary and testis were collected to detect the mRNA expressions of reproduction-related genes with real-time fluorescence quantitative PCR, and the hypothalamus and testes were excised for tissue GnRH detection and morphological observation, respectively. Compared to intact controls, immunocastration reduced (p<0.05) serum concentrations of testosterone, LH and FSH, and the GnRH content in the median eminence, reduced the weight of the hypohysis (p<0.01), and induced testicular atrophy (suppression of spermatogenesis). Furthermore, mRNA expression of GnRH in the hypothalamus, GnRH receptor, LH-β and FSH-β in the pituitary, LH receptor and FSH receptor in the testes, and genes in sex steroid feedback loops (androgen receptor (AR), kisspeptin encoded gene (Kiss-1) and kisspeptin receptor (GPR54) in the hypothalamus were decreased in immunocastrated rats compared to intact controls (p<0.05). Similarly, surgical castration reduced GnRH in the median eminence as well as mRNA expression of GnRH, AR, Kiss-1 and GPR54 in the hypothalamus (p<0.05). These results suggest that active immunization against GnRH reduces GnRH synthesis in the hypothalamus by decreasing androgen-androgen receptor-Kisspeptin-GPR54 signaling pathways and causes dysfunction of the pituitarytesticular axis. Both the loss of GnRH synthesis and dysfunction of the pituitary- testicular axis seem to be mechanisms underlying immunocastration.

키워드

GnRH Anti-GnRH immunization GnRH synthesis Reproductive axis Rat

저자

  • Xing-fa Han [ Isotope Research Lab, Sichuan Agricultural University ]
  • Xian-yin Zeng [ Isotope Research Lab, Sichuan Agricultural University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    한국동물번식학회 한중일 심포지엄
  • 간기
    연간
  • 수록기간
    2014~2014
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 한국동물번식학회 한중일 심포지엄 The 3rd Korea-China-Japan Joint Symposium on Animal Reproduction

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.