Earticle

초록

영어

Actin-related protein 2/3 (Arp2/3) complex plays a central role in the de novo nucleation of filamentous actin as branches on existing filaments. Small molecule inhibitor CK-666 bind to Arp2/3 complex and inhibit nucleation, but their modes of action are unknown. Here, we shed light on the functional aspects of Arp2/3 by CK-666 inhibitor on porcine early embryo development. Our data indicated that CK-666 destabilizes the complex, blocking movement of the Arp2 and Arp3 subunits, causing actin degradation and failure of cell division which results in failure of early embryo development. Our results also shows there is decrease in mRNA expression of ICM and trophectoderm related genes such as Sox2, Pou5f1, Nanog. These results provided key insights into the relationship between early embryo dvelopement and critical role of Arp2/3 complex.

키워드

저자

• Ying-Hua Li [ National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University ]
• Madhusmita Dhupal [ National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University ]
• Yong-Nan Xu [ National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636