Earticle

초록

영어

JMY (junction-mediating and regulatory protein) is a transcriptional co-factor of p53, little information is available on porcine oocyte. Herein we demonstrated that JMY robustly expressed in oocytes, where it was localized predominantly in cytoplasm, and JMY mRNA expressed largely from germinal vesicle to metaphase I stage, and gradually decreased during metaphase II (MII) stages. Depletion of JMY by double-stranded RNA (dsRNA) influenced actin and Arp2/3 expression, further blocking maturation of porcine oocyte in vitro. The p53 transcription JMY responsed in DNA damage conditions, undergoing nuclear accumulation and mediated p53 expression. On the basis of our results, we proposed that p53 co-factor JMY regulated actin and Arp2/3 linked in vitro meiotic maturation in porcine oocyte and related to DNA damage with the p53 response.

키워드

저자

• Zili Lin [ National Research laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development,Chungbuk National University ]
• Suk Namgoong [ National Research laboratory of Molecular Embryology, Department Animal Sciences, Chungbuk National University ]
• Xiangshun Cui [ National Research laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development,Chungbuk National University ]
• Namhyung Kim [ National Research laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development,Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636