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Comparison of Actin Nucleation in Early Stage Mouse and Bovine Oocytes followed by Vitrification and Slow Freezing

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 38 No 2 Supplement (2014.06)바로가기
  • 페이지
    pp.49-49
  • 저자
    Young-Tae Heo, Yu-Jin Jo, Hak-Chul Kim, Joon-Gyo Lim, Sang-Gil Jeon, Chang-Eun Park, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A227113

원문정보

초록

영어
The cryopreservation of unfertilized oocytes is valuable for both livestock and human.
However, freezing procedure to oocyte induce many kinds damages such as cytoskeleton
disorganization, and chromosome and DNA abnormalities. Especially, during mammalian
oocyte meiotic maturation, a process called oocyte polarization occurs, which
leads to a unique asymmetric division. Actin play key roles during oocyte polarization
experiences several steps including spindle migration, spindle anchoring and cortical
reorganization. However, how chilling stress affect to actin nucleation in oocytes is
poorly studied. In this study, we investigated expressions and feature of Arp2/3 (actin
activators), WAVE2 and JMY (nucleation promoting factors) in MІІ stage oocytes after
different freezing procedure in oocytes. Results indicated that survival, IVF, developmental
rates of vitrified-thawed oocytes were significantly higher than those of slow
freezed oocytes. Especially, expressions of Arp2/3, WAVE2 and JMY in vitrified-thawed
oocytes were similar with normal oocytes. However, expression of actin nucleation related
genes in slow freezed group was significantly higher or lower than normal
oocytes. Our results may suggested that slow freezing procedure to oocyte induced
abnormal actin neucleation results in low survival and developmental rates.

키워드

Oocytes Vitrification Slow freezing Actin nuecleation

저자

  • Young-Tae Heo [ Department of Animal Sciences, Chungbuk National University ]
  • Yu-Jin Jo [ Department of Animal Sciences, Chungbuk National University ]
  • Hak-Chul Kim [ Department of Animal Sciences, Chungbuk National University ]
  • Joon-Gyo Lim [ Min Byeong Yeol Obstetrics and Gynecology ]
  • Sang-Gil Jeon [ Min Byeong Yeol Obstetrics and Gynecology ]
  • Chang-Eun Park [ Department of Biomedical Laboratory Science, Namseoul University ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement

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