Earticle

초록

영어

After somatic cell nuclear transfer (SCNT), epigenetic state of a differentiated donor cell nucleus must be reversed to the embryonic state. Incomplete epigenetic reprogramming is thought to be causing the low cloning efficiency. The present study was carried out to investigate the effects and mechanism of scriptaid, a novel histone deacetylase inhibitor (HDACi), on the in vitro development of porcine SCNT embryos. We found that treating SCNT embryos with 300 or 500 nM scriptaid for 20 h increased the development of embryos to the blastocyst stage and total cell numbers in per blastocyst( p<0.05). Apoptosis in blastocyst was decreased (p<0.05) in the presence of scriptaid. Scriptaid treatment significantly (p<0.05) increased the levels of H3-acK9 and 5-hmc, and the levels of H3-m3K9 and 5-mc were decreased at the pronuclear stage. Expression level of mir-152 was significantly (p<0.05) increased in scriptaid treated embryos. Moreover, treating embryos with 300 nM scriptaid increased the expression level of the genes that play important roles during embryonic development (OCT4 and CDX2). Additionally, mRNA expression of Dnmt1, Cas3 and Bak were significantly decreased and Bcl-xL was increased in scriptaid treated group compared to control. In conclusion, scriptaid improves the developmental capacity, prevent apoptosis through improving the epigenetic reprogramming in porcine SCNT embryos.

키워드

저자

• Shuang Liang [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
• Ming-Hui Zhao [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
• Jung-Woo Kwon [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
• Seon-Hyang Kim [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
• Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636