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Structural Basis of Emi2 Recognition by Polo-Box Domain of Polo-like Kinase 1 and Effects of Structure-Derived Antagonist in Oocyte Maturation and Fertillization

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 38 No 2 Supplement (2014.06)바로가기
  • 페이지
    pp.25-25
  • 저자
    Jia-Jia Lin, Young-Hyun Han, Jung-Woo Kwon, Yong-Nan Xu, Yi-Bo Luo, Yu-Jin Jo, Chang-Eun Park, Jung-Kyu Baang, Suk Namgoong, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A227099

원문정보

초록

영어
In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.

키워드

plk1 emi2 CSF Protein structure Chemical inhibitors Oocyte maturation Fertillization

저자

  • Jia-Jia Lin [ Department of Animal Sciences, Chungbuk National University ]
  • Young-Hyun Han [ Department of Animal Sciences, Chungbuk National University ]
  • Jung-Woo Kwon [ Department of Animal Sciences, Chungbuk National University ]
  • Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University ]
  • Yi-Bo Luo [ Department of Animal Sciences, Chungbuk National University ]
  • Yu-Jin Jo [ Department of Animal Sciences, Chungbuk National University ]
  • Chang-Eun Park [ Departmet of Biomedical Laboratory Science, Namseoul University ]
  • Jung-Kyu Baang [ Korean Basic Science Institute ]
  • Suk Namgoong [ Department of Animal Sciences, Chungbuk National University ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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