Earticle

초록

영어

The potential benefits of generating and using transgenic cattle range from im-provements in agriculture to the production of large quantities of pharmaceutically rele-vant proteins. Previous studies have attempted to produce transgenic cattle and other livestock by pronuclear injection and somatic cell nuclear transfer, but these approa-ches have been largely ineffective; however, a third approach, lentivirus-mediated trans-genesis, has successfully produced transgenic livestock. In this study, we generated transgenic (TG) Korean native cattle using perivitelline space injection of viral vectors, which expressed enhanced green fluorescent protein (EGFP) systemically. Two different types of lentiviral vectors derived from feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) carrying EGFP were injected into the perivitelline space of MII oocytes. EGFP expression at 8-cell stage was significantly higher in the FIV group compared to the HIV group (47.5±2.2% vs. 22.9±2.9%). Eight- cell embryos that expressed EGFP were cultured into blastocysts and then transferred into 40 heifers. Ten heifers were successfully impregnated and delivered 10 healthy calves. All of these calves expressed EGFP as detected by in vivo imaging, PCR and Southern blotting. In addition, we established an EGFP-expressing cell line from TG calves, which was followed by nuclear transfer (NT). Recloned 8-cell embryos also expressed EGFP, and there were no differences in the rates of fusion, cleavage and development between cells derived from TG- and non-TG calves, which were subsequently used for NT. These results illustrate that FIV-based lentiviruses are useful for the production of TG cattle. Moreover, our established EGFP cell line can be used for additional studies that involve induced pluripotent stem cells.

키워드

저자

• Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University ]
• Sang-Jun Uhm [ Department of Animal Science and Biotechnology Sangji Youngseo College ]
• Bon-Chul Koo [ Department of Physiology, Catholic University of Daegu School of Medicine ]
• Mo-Sun Kwon [ Department of Physiology, Catholic University of Daegu School of Medicine ]
• Ji-Yeol Roh [ Department of Animal Sciences, Chungbuk National University ]
• Jung-Seok Yang [ Department of Animal Science and Biotechnology, Sangji Youngseo College ]
• Hyun-Yong Choi [ Department of Animal Sciences, Chungbuk National University ]
• Young-Tae Heo [ Department of Animal Sciences, Chungbuk National University ]
• Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University ]
• Joon-Ho Yoon [ Department of Animal Resources Community, KongJu National University ]
• Dae-Hwan Ko [ Department of Animal Science and Biotechnology, Sangji Youngseo College ]
• Teoan Kim [ Department of Physiology, Catholic University of Daegu School of Medicine ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636