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Human Six Reprogramming Factors can Reprogram Porcine Somatic Cells into Naïve-Like Induced Pluripotent Stem Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 37 No 2 Supplement (2013.06)바로가기
  • 페이지
    pp.153-153
  • 저자
    DaeJin Kwon, Won Gun Noh, Keon Bong Oh, Seongsoo Hwang, Sun A Ock, Seok-ki Im, Jin-Hoi Kim, Jin-Ki Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A226574

원문정보

초록

영어
Porcine pluripotent stem cells have important in modeling embryonic development and disease processes in biomedical research, especially to transplantation medicine, immunology and circulatory system. However, despite years of effort, the pluripotency machinery of porcine PSCs has not been demonstrated clearly. Porcine induced plu-ripotent stem cells (piPSCs) were generated using combination of six human tran-scriptional factors under stem cell culture condition. To confirm dependency of these piPSCs, they were separately cultured in stem cell media supplemented with i) 20 ng/ml LIF, ii) 20 ng/ml bFGF, iii) 20 ng/ml BMP4, iv) 20 ng/ml LIF and Su5402, or v) 20 ng/ml bFGF and Jak inhibitor I, for 48 h, respectively. Upon bFGF treated groups, colonies show signs of differentiation with dispersing colony morphology and losing alkaline phosphatase activity. In contrast, colonies with tightly expanded and strong AP activity are shown in LIF or BMP4 treated groups. Western blotting shows phospho- Stat3 and phospho-Smad1/5 are significantly suppressed by Jak inhibitor I. We con-clude that piPSCs generated by six human pluripotent factors, are dependent on LIF or BMP4 to sustain self-renewal and pluripotency.

키워드

Porcine induced pluripotent stem cells (piPSCs) Self-renewal Pluripotency Hu-man six reprogramming factors

저자

  • DaeJin Kwon [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Won Gun Noh [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Sun A Ock [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Seok-ki Im [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Jin-Hoi Kim [ Department of Animal Biotechnology, Konkuk University ]
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 37 No 2 Supplement

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