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A Simple Culture for Gonadal Germ Cells from 7-day-old Korean Native Chicken (Ogye Breed) Embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 37 No 2 Supplement (2013.06)바로가기
  • 페이지
    pp.148-148
  • 저자
    Hyun Kim, Yeoung-Gyu Ko, Sung Bok Choi, Yoon Jung Do, Jae Yong Han, Dong Hun Kim, Soo Bong Park, Hwan-Hoo Seong, Sung Woo Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A226569

원문정보

초록

영어
Recent technological progress in producing germline chimeras has provided an important means of studying genetic resource conservation, and transgenics production. Primordial germ cells (PGCs) are called gonadal germ cells (GGCs), after migration to the gonadal ridge. GGCs ultimately differentiate into spermatogonia in the testes or oogonia in the ovaries. Germline chimeras can be produced in domestic chicken by transferring GGCs collected from the embryonic gonad into the bloodstream of 2-dayold recipient embryos. However, it is diffcult to purify GGCs from the majority number of somatic cells after digesting embryonic gonads using proteinases such as trypsin and EDTA. The aim of the present study was to develop a simple method for isolating GGCs from the gonads of 7-day-old chick embryos. A simple method for isolating viable GGCs was developed in the domestic chicken. Developing gonads were isolated from 7-day-old chick embryos and cultured for up to 24 hours at 38.5℃ in phosphate buffered saline without Ca+ and Mg+ (PBS [-]). A discharge of GGCs from the gonad was observed within 40 minutes after introducing the embryonic gonad into PBS [-], and the number of discharged GGCs increased until 12 hours of incubation. The purity of the GGCs (number of discharged GGCs/total number of discharged cells) was approximately 48% for the initial 1 hours of incubation and decreased thereafter. These results indicate that high-purity, viable GGCs can be collected by simply introducing isolated developing gonads into PBS [-]. The results provide an alternative means for producing germ line chimeras to conserve genetic resources and to produce transgenic avian species.

키워드

Chicken Gonadal germ cells Isolation PBS[-]

저자

  • Hyun Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Yeoung-Gyu Ko [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Sung Bok Choi [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Yoon Jung Do [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Jae Yong Han [ WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University ]
  • Dong Hun Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Soo Bong Park [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Hwan-Hoo Seong [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Sung Woo Kim [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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