Earticle

초록

영어

Phellinus xeranticus is an medicinal mushroom belongs to Family Hymenochaetaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the antioxidant, anti- inflammatory and anti-acetylcholinesterase activities of methanol and hot water extracts prepared from fruiting bodies of Phellinus xeranticus. Besides measuring of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, a reducing power and a chelating activity on ferrous ions were also measured to evaluate the antioxidant activity of the extracts. To measure the anti-inflammatory activities of the extracts, nitric oxide(NO) production from lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells and carrageenan-induced acute hind paw edema of rats were investigated. The results showed that the extracts have excellent DPPH scavenging and chelating activity on the ferrous ions compared with positive controls. The nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells were decreased as the concentration of the mushroom extracts increased. Significant reduction of paw edema of rats were observed at 2~6 h after treatment of methanol and hot-water extracts with 50 mg/kg concentration to the rats which are induced acute hind paw edema by carrageenan administration. The anti-acetylcholinesterase activity of the methanol extract of the mushroom showed 83.34% inhibition on AcHE which is lower than that of positive control galanthamine. The experimental results suggested that methanol and hot-water extracts of Phellinus xeranticus fruiting bodies might be used for good sources of antioxidant, anti-inflammatory and anti-acetylcholinesterase agents.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  성분의 추출 및 분리
  총 폴리페놀 함량 측정
  총 플라보노이드 함량 측정
  세포배양
  세포의 생존율 측정
  DPPH 라디칼 소거 활성 측정
  환원력 활성 측정
  철 이온 제거능
  Nitrite(NO) 생성 저해효과 측정
  Carrageenan에 의한 부종유발 및 소염효과의 측정
  AChE의 활성 저해효과 측정
  통계 처리
 결과 및 고찰
  총 폴리페놀 및 플라보노이드 함량 측정
  세포독성 분석
  DPPH 라디칼 소거활성
  철 이온 제거능
  환원력
  NO 생성 저해효과
  Carrageenan에 의한 부종유발 및 항염 효과의 측정
  Acetylcholinesterase 저해 활성
 적요
 감사의 말씀
 참고문헌

키워드

저자

• Trung Kien Nguyen [ 인천대학교 생명과학기술대학 생명과학부 ]
• 신도빈 [ Do Bin Shin | 인천대학교 생명과학기술대학 생명과학부 ]
• 이경림 [ Kyung Rim Lee | 인천대학교 생명과학기술대학 생명과학부 ]
• 신평균 [ Pyung Gyun Shin | 원예특작과학원 버섯과 ]
• 정종천 [ Jong Chun Cheong | 원예특작과학원 버섯과 ]
• 유영복 [ Young Bok Yoo | 원예특작과학원 버섯과 ]
• 이민웅 [ Min Woong Lee | 동국대학교 ]
• 진가헌 [ Ga-Heon Jin | 신흥대학교 안경과학과 ]
• 김혜영 [ Hye Young Kim | 신성대학교 임상병리과 ]
• 임경환 [ Kyung Hoan Im | 인천대학교 생명과학기술대학 생명과학부 ]
• 이태수 [ Tae Soo Lee | 인천대학교 생명과학기술대학 생명과학부 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635