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The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

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  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    한국버섯학회지 바로가기
  • 통권
    제11권 제4호 (2013.12)바로가기
  • 페이지
    pp.194-200
  • 저자
    Min Woong Lee, Kwang Chun Chang, Do Bin Shin, Kyung Rim Lee, Kyung Hoan Im, Ga-Heon Jin, Pyung Gyun Shin, Yong Mei Xing, Juan Chen, Shun Xing Guo, Tae Soo Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A226494

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초록

영어
Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were 20oC and pH 4, while optimal conditions for mycelial growth of A. mellea were 25oC and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at 20oC under dark condition.

목차

ABSTRACT
 Introduction
 Materials and Methods
  Fungal strains
  Culture conditions for mycelial growth of P.
  Effect of nutrient source
  Effect of nutrient source for sclerotium formation
  Statistical analysis
 Results and Discussion
  Culture conditions for mycelial growth of P.
  Nitrogen sources
  Effect of nutrient sources and pH values for sclerotium formation
 Acknowledgement

키워드

Armillaria mellea Polyporus umbellatus Sclerotium Symbiosis

저자

  • Min Woong Lee [ Department of Life Science, Dongguk University, Seoul 100-715, Korea ]
  • Kwang Chun Chang [ Department of Life Science, Dongguk University, Seoul 100-715, Korea ]
  • Do Bin Shin [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
  • Kyung Rim Lee [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
  • Kyung Hoan Im [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
  • Ga-Heon Jin [ Department of Ophthalmic Optics, Shinheung University 95 Hoam-ro, Uijeongbu, Gyeongggi 480-701, Korea ]
  • Pyung Gyun Shin [ Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Korea ]
  • Yong Mei Xing [ of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, eijing 100094, People's Republic of China. ]
  • Juan Chen [ Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, People's Republic of China. ]
  • Shun Xing Guo [ Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, People's Republic of China. ]
  • Tae Soo Lee [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    한국버섯학회지 [Journal of MUSHROOMS]
  • 간기
    계간
  • pISSN
    1738-0294
  • eISSN
    2288-8853
  • 수록기간
    2003~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 525 DDC 635

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