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Effects of Essential Amino Acids on the In Vitro Development of Porcine Parthenogenetic Embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 37 No 2 Supplement (2013.06)바로가기
  • 페이지
    pp.115-115
  • 저자
    Yeon-Ik Jeong, Chi-Hun Park, Dong-Kyung Lee, Hee-Jin Song, Yeun-Wook Kim, Yeon-Woo Jeong, Sang-Hwan Hyun, Hwan-Yul Yong, Woo-Suk Hwang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A226101

원문정보

초록

영어
Amino acids have been regarded as beneficial for the development of preimplantation embryos and acted critically in the in vitro culture of porcine embryos. However, some reviews of emerging literatures show that supplementation of essential amino acids (EAAs) to in vitro culture medium was improved less than usually expected. The present study was designed to investigate the effects of EAAs on the in vitro development of porcine preimplantation embryos after parthenogenetic activation. The results showed that supplementation of EAAs to porcine zygote medium-3 (PZM-3) with 1:50 significantly decreased the blastocyst rate of parthenogenetic embryos (12.8±4.0 vs. 25.6±4.2) although cleavage rate was not different (p<0.05). On the other hand, the distribution pattern of total cell number of the blastocysts varied widely while the average number has not shown significant difference. Real-time qPCR analysis revealed that EAAs have turned over transcription pattern of imprinted genes: the relative abundance of H19 transcript was enhanced and level of IGF2r transcript was decreased significantly (p<0.05). Interestingly, gene expression of mTOR, activated by some essential amino acids, was significantly up-regulated (p<0.05). In conclusion, these results indicate that the presence of essential amino acids in the in vitro culture medium might impair embryo development due to mRNA turnover of imprinted genes, but could possibly play an important role at the implantation stage because development of protrusive trophoblast activity requires some amino acids-dependent mTOR signaling in early blastocysts.

키워드

Essential amino acids In vitro development Porcine Parthenogenetic embryos

저자

  • Yeon-Ik Jeong [ Sooam Biotech Research Foundation ]
  • Chi-Hun Park [ Sooam Biotech Research Foundation ]
  • Dong-Kyung Lee [ College of Veterinary Medicine, Chungbuk National University ]
  • Hee-Jin Song [ Sooam Biotech Research Foundation ]
  • Yeun-Wook Kim [ Sooam Biotech Research Foundation ]
  • Yeon-Woo Jeong [ Sooam Biotech Research Foundation ]
  • Sang-Hwan Hyun [ College of Veterinary Medicine, Chungbuk National University ]
  • Hwan-Yul Yong [ Sooam Biotech Research Foundation ]
  • Woo-Suk Hwang [ Sooam Biotech Research Foundation ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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