Earticle

초록

영어

Meiotic maturation in many species is initiated by the activation of maturation-promoting factor (MPF) with concomitant inactivation of counteracting phosphatases, most notably protein phosphatase 2A (PP2A). Recently, Greatwall (Gwl) has been identified as a cell cycle regulator that inhibits PP2A activity. In this study, we demonstrate that Gwl is required for meiotic maturation in porcine oocytes. Gwl is expressed from germinal vesicle (GV) to metaphase II (MII) stages of porcine oocytes and dramatically decreases with the progression of the meiotic cell cycle. Gwl is initially localized in the nucleus of GV oocytes and is associated with spindle fibers following GV breakdown (GVBD). Depletion of Gwl inhibits or delays meiotic maturation secondary to defects in chromosome congression and spindle formation. Conversely, overexpression of Gwl overcame meiotic arrest and initiated progression to the MII stage. However, these oocytes had severe spindle defects. Furthermore, MII oocytes depleted of Gwl progressed to pronuclear formation. Taken together, our data demonstrate that Gwl is required not only for meiotic maturation but also for maintenance of MII arrest in porcine oocytes.

키워드

저자

• Ying-Hua Li [ Department of Animal Sciences, Chungbuk National University ]
• Hyoeun Kang [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University ]
• Young-Tae Heo [ Department of Animal Sciences, Chungbuk National University ]
• Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University ]
• Jeong Su Oh [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636