Earticle

현재 위치 Home

Effects of Growth Differentiation Factor 9 and Bone Morphogenic Protein 15 on the In Vitro Maturation of Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 37 No 2 Supplement (2013.06)바로가기
  • 페이지
    pp.23-23
  • 저자
    Zi-Li Lin, Ying-Hua Li, Yong-Nan Xu, Suk Namgoong, Xiang-Shun Cui, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A226015

원문정보

초록

영어
Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are members of the transforming growth factor β (TGF-β) family, and their roles in oocyte maturation and cumulus expansion are well known in the mouse and human, but not in the pig. We investigated GDF9 and BMP15 expression in porcine oocytes during in vitro maturation. A significant increase in the mRNA levels of GDF9 and BMP15 was observed at germinal vesicle breakdown, with expression levels peaking at metaphase I (MI) but decreasing at metaphase II (MII). GDF9 and BMP15 protein localized to the oocyte cytoplasm. While treatment with GDF9 and BMP15 increased cumulus expansion and the expression of genes involved in both oocyte maturation (c-mos, cyclinb1, and cdc2) and cumulus expansion (has2, ptgs2, ptx3, and tnfaip6). SB431542 (a TGFβ–GDF9 inhibitor) decreased meiotic maturation at MII. Following parthenogenetic activation, the percentage of blastocysts in SB431542 treatment was lower than in the control (74.4% and 41.3%, respectively). Treatment with GDF9 and BMP15 also increased the mRNA levels of maternal genes such as c-mos (a regulatory subunit of mitogen-activated protein kinase (MAPK)), and cyclinb1 and cdc2 (regulatory subunits of maturation/M phase promoting factor (MPF)); however, SB431-542 significantly decreased their mRNA levels. These data were supported by poly (A)-test PCR and protein activity analyses. Our results show that GDF9 and BMP15 function in cumulus expansion and that they stimulate MPF and MAPK activity in porcine oocytes during in vitro maturation.

저자

  • Zi-Li Lin [ Department of Animal Sciences, Chungbuk National University ]
  • Ying-Hua Li [ Department of Animal Sciences, Chungbuk National University ]
  • Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University ]
  • Suk Namgoong [ Department of Animal Sciences, Chungbuk National University ]
  • Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 37 No 2 Supplement

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.