Earticle

초록

영어

Amyloid beta (Aβ) peptide has been implicated in the pathogenesis of Alzheimer’s disease and has been reported to induce apoptotic death in cell culture. Cysteine Proteases, a family of enzymes known as caspases, mediate cell death in many models of apoptosis. In the present study, we examined the caspase activity and cell death in Aβ -treated SHSY5Y cells, as an attempt to elucidate the relationship between the type of caspase and Aβ-induced cell death. Aβ at 20 μM induce activation of caspase-3, 8 and 9 activity, but not the caspase-1. Caspase-3, 8 and 9 were processed by Ab treatment, consistent with the activity assay. Inhibition of the caspase activities by the selective inhibitors, however, marginally affected the cell death induced by Aβ. Taken together, the results indicate that Aβ-induced cell death may be independent of caspase activity and rather, the enzymes might be activated as a result of the cell death.

목차

Abstract
 1. Introduction
 2. Experimental Section
  2.1. Reagents
  2.2. Preparation of Aβ
  2.3. Cell Culture and Cell Death Assay
  2.4. Caspase Activity Assay
  2.5. Western Blot Analysis
 3. Results and Discussion
  3.1 Aβ Induces Caspase-3, 8, 9 Activation, not Caspase-1
  3.2. Caspase Inhibition Does not Prevent Aβ-Induced Cell Death
 4. Conclusion
 References

키워드

저자

• Pham Thi Dieu Tuyet [ Department of Biotechnology, Hanoi University of Science and Technology, University of Hanoi, Vietnam ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]

• 간기

  계간

• pISSN

  2005-1042

• 수록기간

  2008~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 405 DDC 505