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Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 1 (2006.03)바로가기
  • 페이지
    pp.41-45
  • 저자
    Naruse Kenji, Kim Hong-Rye, Shin Young-Min, Chang Suk-Min, Lee Hye-Ran, Tarte Vaishali, Quan Yan-Shi, Kim Beak-Chul, Park Tae-Young, Choi Su-Min, Park Chang-Sik, Jin Dong-Il
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A22250

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초록

영어
Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10μgml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10μgml cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to 6.25μM calcium ionophore for 2 min with 10μgml cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Porcine oocytes Cycloheximide Electrical stimulation Parthenogenetic development In vitro culture

저자

  • Naruse Kenji [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Kim Hong-Rye [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Shin Young-Min [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Chang Suk-Min [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Lee Hye-Ran [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Tarte Vaishali [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Quan Yan-Shi [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Kim Beak-Chul [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Park Tae-Young [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Choi Su-Min [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Park Chang-Sik [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Jin Dong-Il [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 30 No 1

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