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Sperm Fertility of Transgenic Boar Harboring hEPO Gene is Decreased

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 1 (2006.03)바로가기
  • 페이지
    pp.27-34
  • 저자
    Park Chun-Gyu, Kim Sung-Woo, Lee Poong-Yeon, Han Joo-Hee, Lee Hyun-Gi, Byun Sung-June, Yang Boh-Suk, Lee Chang-Hyung, Lee Hoon-Taek, Chang Won-Kyong, Park Jin-Ki
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A22248

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원문정보

초록

영어
This study was conducted to compare the reproduction ability of the wild type boar and recombinant human erythropoietin (hEPO) transgenic boar semen. Ejaculated boar semen was analyzed by flow cytometry, Elisa and IVF methods. In experiment 1, flow cytometric analysis showed that the live sperm ratio of transgenic boar sperm significantly lower (P<0.05) than that of wild type boar after incubation at 20, 22, 24 and 26 hr. In experiment 2, the presence and levels of various cytokines (IL-6, IL-10 and TNF-α) to related animal reproduction in the seminal and blood plasma were examined using specific enzyme immunoassay. There was no significant difference between both groups. In experiment 3, the fertilizing capacity and developmental ability of both boar sperm were compared. The transgenic boar sperm had a significantly low capacity of penetration, sperm-zona binding, embryo development, and blastocyst formation compared to wild type sperm (P<0.05). These results suggest that transgenic boar sperm harboring hEPO gene has low sperm viability than wild type boar, and it is a reason to decrease of fertility and litter size.

목차

INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

hEPO transgenic pig Semen flow cytometry Cytokine IVF

저자

  • Park Chun-Gyu [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Kim Sung-Woo [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Lee Poong-Yeon [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Han Joo-Hee [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Lee Hyun-Gi [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Byun Sung-June [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Yang Boh-Suk [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Lee Chang-Hyung [ Department of Animal Science, Korea National Agricultural College, RDA ]
  • Lee Hoon-Taek [ Animal Resources Research Center, Konkuk University ]
  • Chang Won-Kyong [ Animal Biotechnology Division, National Livestock Research Institute ]
  • Park Jin-Ki [ Animal Biotechnology Division, National Livestock Research Institute ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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