Earticle

초록

영어

Nuts are one of the most common sources of allergies in individuals of all ages. In order for a particular protein to render an allergic reaction, it must resist proteolytic digestion by intestinal enzymes. In this study, three well-known allergenic nuts, almonds, cashew nuts, and peanuts, were used as samples, and enzyme digestion with Bacillus protease and porcine pepsin was tested. A proteomic approach using two-dimensional gel electrophoresis and an MS/MS analysis was applied to visualize and identify the proteins that were resistant to enzyme digestion. Among the 150 protein spots tested, 42 proteins were assigned functions. Due to the lack of genomic databases, 41% of the identified proteins were grouped as hypothetical. However, 12% of them were well-known allergens, including AraH. The remainder were grouped as storage, enzymes, and binding proteins.

키워드

저자

• Ilyn L. Santos [ Major in Food Biomaterials, Kyungpook National University ]
• Ju-Young Lee [ Major in Food Biomaterials, Kyungpook National University ]
• Yujin Youm [ Major in Food Biomaterials, Kyungpook National University ]
• Jinkyu Lim [ Major in Food Biomaterials, Kyungpook National University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  경북대농학지

• 간기

  계간

• pISSN

  2287-271X

• eISSN

  2288-0356

• 수록기간

  ~2014

• 십진분류

  KDC 525 DDC 635