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Original Article

Recombinant Protein Expression and Purification of the Human HMTase MMSET/NSD2

첫 페이지 보기
  • 발행기관
    경북대학교 농업생명과학대학 바로가기
  • 간행물
    경북대농학지 바로가기
  • 통권
    Vol. 31 No. 3 (2013.09)바로가기
  • 페이지
    pp.157-164
  • 저자
    Masayo Morishita, Damiaan Mevius, Yunpeng Shen, Eric di Luccio
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A221260

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원문정보

초록

영어
Chromatin remodelers that include histone methyl transferases (HMTases) are becoming a focal point in cancer drug development. The NSD family of three HMTases, NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L are bona fide oncogenes found aberrantly expressed in several cancers, suggesting their potential role for novel therapeutic strategies. Several histone modifiers including HMTase have clear roles in human carcinogenesis but the extent of their functions and regulations are not well understood, especially in pathological conditions. The extents of the NSDs biological roles in normal and pathological conditions remain unclear. In particular, the substrate specificity of the NSDs remains unsettled and discrepant data has been reported. NSD2/MMSET is a focal point for therapeutic interventions against multiple myeloma and especially for t(4;14) myeloma, which is associated with a significantly worse prognosis than other biological subgroups. Multiple myeloma is the second most common hematological malignancy in the United States, after non-Hodgkin lymphoma. Herein, as a first step before entering a pipeline for protein x-ray crystallography, we cloned, recombinantly expressed and purified the catalytic SET domain of NSD2. Next, we demonstrated the catalytic activities, in vitro, of the recombinantly expressed NSD2-SET on H3K36 and H4K20, its biological targets at the chromatin.

키워드

Epigenetic therapy of cancer Histone-lysine methyltransferase NSD2 MMSET Recombinant protein expression

저자

  • Masayo Morishita [ College of Agriculture and Life Science, School of Food Biomaterials, Kyungpook National University ]
  • Damiaan Mevius [ College of Agriculture and Life Science, School of Food Biomaterials, Kyungpook National University ]
  • Yunpeng Shen [ College of Agriculture and Life Science, School of Food Biomaterials, Kyungpook National University ]
  • Eric di Luccio [ College of Agriculture and Life Science, School of Food Biomaterials, Kyungpook National University ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    경북대학교 농업생명과학대학
  • 분야
    농수해양>농학

간행물

  • 간행물명
    경북대농학지
  • 간기
    계간
  • pISSN
    2287-271X
  • eISSN
    2288-0356
  • 수록기간
    ~2014
  • 십진분류
    KDC 525 DDC 635

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