Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti- inflammatory, and melanin synthesis inhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extracts showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions compared with the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS- induced RAW 264.7 macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h after administration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg of indomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with 100 μg/mL of the methanol and hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, the experimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used for good sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.
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ABSTRACT 서론 재료 및 방법 실험재료 성분의 추출 및 분리 총 폴리페놀 함량 측정 총 플라보노이드 함량 측정 DPPH 라디칼 소거 활성 측정 철 이온 제거능 측정 환원력 활성 측정 추출물의 세포 독성 측정 Nitrite (NO) 생성 저해효과 측정 Carrageenan에 의한 흰쥐 뒷발의 부종유발 및 소염효과의 측정 티로시나아제 활성 저해능 측정 Melanoma B16/F10 세포내 멜라닌의 생성억제 측정 통계 처리 결과 및 고찰 총 폴리페놀 및 플라보노이드의 함량 DPPH 라디칼 소거활성 철 이온 제거능 환원력 추출물의 세포독성 NO 생성 저해 Carrageenan에 의한 부종유발 및 항염 효과 티로시나아제 활성 저해 Melanoma B16/F10 세포내 멜라닌의 생성 저해 적요 감사의 글 참고문헌