Earticle

초록

영어

The primary objective of the present study is the characterization of the somatic hybrids of dikaryon- monokaryon (dimono) crosses in mushroom breeding. We employed this technique for developing superior strains from Pleurotus ostreatus strains with 48 intraspecific hybrids of 12 combinations between six P. ostreatus strains and one P. florida strain. The results on the experiments of hybridization rate, nuclear DNA patterns, and colors and morphology of fruit-bodies, are presented. In di-mono crosses, somatic hybrids among Pleurotus strains showed 100% of crossability as seen in those between P. ostreatus and P. florida strains indicating that the nuclei of a dikaryon is inferred to be migrated to a recipient. 87.5% of the somatic hybrids among Pleurotus strains were similar to the donor dikaryons, and 12.5% of the somatic hybrids presented DNA patterns of both parents. In 16.6% of di-mono crosses between P. ostreatus and P. florida, the nuclear DNA patterns of all hybrids showed the same or similar patterns compared to the donor dikaryons. 70.9% of the hybrids between P. ostreatus and P. ostreatus were similar to the donor dikaryons and 12.5% of them presented the DNA patterns of both parents. 79.2% of fruiting body morphology of the hybrids among Pleurotus strains were similar to the dikaryons and 20.8% of them were similar to both parents. Interestingly, the morphology of all dikaryons were dissimilar each other. All hybrid strains between dikaryon P. florida and monokaryon P. ostreatus showed the fruiting body of which colors were similar to those of the dikaryon, while the hybrids between dikaryon P. ostreatus and monokaryon P. florida were showed the combined colors of both parents. Therefore, the fruiting body color of P. florida tends to be generally dominant. In conclusion, the present study provides a way to find out and suggest superior hybrid strains using the nuclear DNA patterns of hybrids between Pleurotus strains as well as the characteristics of their fruiting bodies. The advantages of the di-mono crossing are needs to be fully utilized in mushroom breeding because it is an ideal way to develop the superior strains of Pleurotus.

목차

ABSTRACT
 서론
 재료 및 방법
  균주 및 배양
  단포자 분리 및 교배형 검정
  이핵-단핵(di-mono) 계통간 교잡
  DNA 다형성 분석
  유전유사도 분석
  교잡주의 자실체 특성분석
 결과 및 고찰
 적요
 감사의 말씀
 참고문헌

키워드

저자

• 김은정 [ Eun Jung Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 신평균 [ Pyung Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 한영숙 [ Young Sook Han | 성신여자대학교 식품영양학과 ]
• 유영복 [ Young Bok Yoo | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635