Earticle

초록

영어

The hydrated gel hyaluronic acid (HA) comprises repeating units of glucuronic acid and N- acetylglucosamine. HA production has a lot of significance in the present day scenario owing to its clinical applications. Fermentative optimization is carried out in two steps. Statistical optimization of media components for the production of HA using Taguchi and Response Surface Methodology (RSM) was studied using a mutant strain Streptococcus zooepidemicus 3523-7. Initial optimization was carried out using L27 - orthogonal array. The significance of each factor with respect to HA production was identified using Taguchi design (Glucose, Yeast extract, K2HPO4, NaH2PO4, NaCl, NH4Cl, 1M MgSO4 and Trace metal mix (TMM)) and the productivity was increased 1.34 folds (738 to 988 mg/L) over native production medium. The outcome of Taguchi results indicates the glucose, yeast extract, K2HPO4 and NH4Cl are most influencing factors in the production of HA. Final optimization was carried out using RSM (Central Composite Rotatable Design) and the production was significantly enhanced to 1.4 folds (988 to 1386 mg/L). For the first time statistical optimization was carried out using a mutant strain Streptococcus zooepidemicus 3523-7 and high yield (1386 mg/L) of HA was achieved.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Media Preparation and Culture Conditions
  2.2. Optimization of Production Medium Components using One-Factor-at-a-Time Classical Method
  2.3. Optimization of Concentrations of Production Medium Components using L27 - Orthogonal Array
  2.4. Optimization of Concentrations of the Selected Medium Components by Central Composite Design
  2.5. Isolation of Hyaluronic Acid
 3. Results and Discussion
  3.1. One Factor-at-a-time Method
  3.2. Optimization using L27–orthogonal Array
  3.3. Optimization of Concentrations of the Selected Medium Components by RSM
 4. Conclusion
 Acknowledgments
 References

키워드

저자

• Seetha Ram Kotra [ 1Department of Biotechnology, Acharya Nagarjuna University, Guntur–522510, India ]
• T. C. Venkateswarulu [ School of Biotechnology, Vignan University, Guntur – 522213, India ]
• D. John Babu [ School of Biotechnology, Vignan University, Guntur – 522213, India ]
• Y. Sudheer [ Department of Biotechnology, R.V.R. & J.C. College of Engineering, Guntur–522019, India ]
• K. Seetharami Reddy [ Department of Biotechnology, R.V.R. & J.C. College of Engineering, Guntur–522019, India ]
• J. B. Peravali [ Department of Biotechnology, Bapatla Engineering College, Bapatla–522101, India ] Corresponding author
• K. K. Pulicherla [ Advanced Center for Bioseparation Technology, VIT University, Vellore–632014, Tamilnadu, India ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Advanced Science and Technology

• 간기

  월간

• pISSN

  2005-4238

• 수록기간

  2008~2016

• 십진분류

  KDC 505 DDC 605