Sex determination in some reptiles, amphibians, and fishes is known to be influenced greatly by environmental factors. Aromatase is the key enzyme in estrogen production and therefore is a reliable marker of female differentiation. Aromatase cytochrome P450 (P450arom) is the enzyme complex responsible for conversion of androgens to estrogens in vertebrates. Consequently, in some fishes its activity appears critical to ovarian differentiation. Olive flounder (Paralichthys olivaceus) is a commercially important flatfish in which females grow larger than males and sex determination is temperature sensitive. Through cloning of the P450arom gene in ovary and quantitative reverse transcription-polymerase chain reaction, we developed a biomarker for early female differentiation in olive flounder. The deduced amino acid sequence for olive flounder P450arom is similar to other teleosts. The part of a gonad was subjected to sex discrimination experiment using aromatase marker, and the rest part, by histological observation. The result of sex discrimination by using markers developed in this study was absolutely correspondent to the result acquired from histological observation, confirming its high accuracy as an useful marker. Gonadal histology confirmed predictions of sex based on P450arom expression in juvenile flounder, demonstrating that the patterns of P450arom expression observed relate to sex-specific differentiation. Also, in order to apply the method, developed in this study, juvenile (fry) flounder from fish farm were examined, and it revealed that sex discrimination of flounders under early sex differentiation is successful. This research represents a unique approach to assessing sex differentiation in a natural population, and a powerful technique for better understanding mechanisms of flounder sex determination and rapidly defining conditions for controlling sex for aquaculture assessing sex responses using the aromatase marker.
목차
Abstract 서론 재료 및 방법 1. 조직학적 관찰을 통한 넙치의 암수판별 2. 암수판별을 위한 marker 개발 3. 개발된 암수판별 marker를 이용한 넙치 종묘의 암수판별 결과 1. 조직학적 관찰을 통한 넙치의 암수판별 2. 개발된 암수판별 marker를 이용한 넙치 종묘의 암수판별 고찰 요약 사사 참고문헌
제주대학교 해양과학연구소 [Marine Science Institute Jeju National University]
설립연도
1968
분야
농수해양>수산학
소개
해양의 실체와 그 자원의 합리적 이용과 관리방법을 규명하기 위하여 해양물리, 화학, 생물, 지질, 공학등 여러 관련분야에 관한 다양한 연구를 수행하며, 환경의 보전과 보호를 위해서 수질, 대기, 상·하수도, 폐기물, 사회적 환경 등의 연구를 통하여 쾌적하고 청정한 환경을 보전하기 위한 다양한 연구를 수행하고, 또한 해양 및 환경분야의 우수한 인력양성, 국내외 학술교류의 증진, 학·산 협동체제의 확립 등을 통하여 해양과 환경에 관한 기초 및 응용적 학술연구를 종합적으로 수행함을 목적으로 한다.
간행물
간행물명
해양과학연구소 연구논문집 [Bulletin of the Marine Science Institute]