Earticle

초록

영어

Plant expression system for mass production of highly valuable recombinant therapeutic proteins has several advantages over other existing expression systems with economical and safety issues. Immunotherapy of multiple monoclonal antibodies, which can recognize multiple targeting including specific proteins and their glycans highly expressed on the surface of cancer cells, can be an efficient treatment compared to a single targeting immunotherapy using a single antibody. In this study, we have established plant production system to express two different targeting monoclonal antibodies in a single transgenic plant through crossing fertilization between two different transgenic plants expressing anti-colorectal cancer mAb CO17-1A (mAb CO) and anti-breast cancer mAbBR55 (mAb BR), respectively. The F1 seedlings were obtained cross fertilization between the two transgenic parental plants. The presence, transcription, and protein expression of heavy chain (HC) and light chain (LC) genes of both mAbs in the seedlings were investigated by PCR and immunoblot analyses, respectively. The seedlings with presence and transcription of HC and LC genes of both mAbs were selected, and the selected seedlings were confirmed to have relatively stronger density of HC and LC protein bands compared to the transgenic plant expressing only each mAb. N-glycan structure analysis will be conducted to confirm the glycan structure of multiple monoclonal antibodies mAbP CO x BR. These results indicate that the F1 seedling plant with carrying both mAb genes was established. Taken together, plant crossing fertilization can be applied to generate an efficient production system expressing multiple monoclonal antibodies for immunotherapy in a single plant.

저자

• Deuk-Su Kim [ Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University ]
• Jeong-Hwan Lee [ Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University ]
• Kyung-Jin Lee [ Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University ]
• Young-Kug Choo [ Department of Biological Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
• Kyung-A Hwang [ Department of Agrofood Resources, National Academy of agricultural Science, Rural Development Administration, Suwon, Korea ]
• Kisung Ko [ Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614