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Carbohydrate analysis of functional ingredients of plant materials from roots, leaves, stem bark, and seed
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- 2013 한국당과학회 하계학술대회 (2013.08)바로가기
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- 페이지
- pp.48-48
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A212744
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The carbohydrates in natural plant materials obtained from roots, leaves, stem barks, and seed were investigated. All of the plant materials were dried, and their constituents were isolated by room temperature-water or hot-water extraction. Ethanol precipitation was also used to produce solutions containing the constituents for analysis. Mono- and disaccharides were separated using a high-pH anion-exchange chromatography system equipped with a CarboPac PA10 column and an AminoTrap column working at an isocratic NaOH concentration of 20 mM and at a flow rate of 0.5 ml/min at room temperature for 40 min. Carbohydrates were detected by a pulsed amperometric detector. The concentration of each carbohydrate was quantified by calculation from a calibration curve constructed using a standard mixture containing twofold dilutions from 1,600 pmol of each carbohydrate. Enzymatic deglycosylation of the plant extracts was also performed. Namely, each sample was incubated in the presence of 40 μM trypsin and 40 μM chymotrypsin in 10 mM Tris-HCl buffer (pH 8.0) at 37°C for 18 h. N-glycans were then released from glycopeptides after reaction with 0.1 mU of glycoamidaseA in citrate-phosphate buffer (pH 5.0) at 37°C for 18h. Peptides and salts were removed using graphitized carbon cartridges. The released glycans were lyophilized and stored at –20°C. The N-glycan structures were analyzed by normal-phase high-performance liquid chromatography of 2-aminobenzamide-labeled glycans in combination with exoglycosidase digestion, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The functions of the identified and isolated chemical constituents were also investigated.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2013 한국당과학회 하계학술대회
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