Sa-Rang Kim, jong-Tae Park, Jung-Eun Kim, Jae-Han Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A212736
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원문정보
초록
영어
A 4-α-glucanotransferase (DgαGT EC 2.4.1.25, 4αGTase) gene was cloned from Deinococcus geothermalis and expressed in Escherichia coli, then recombinant protein was purified using nickel affinity chromatography. 4αGTase possesses intermolecular and intramolecular glucan transfer activity. 4αGTase have been used to produce cycloamylose(CA) from amylose by intramolecular glucan transfer activity. CA production by DgαGT was compared with those by Thermus scotoductus 4αGTase (TSαGT) and Escherichia coli 4αGTase (MalQ). Optimal CA production conditions were 50℃ and pH 6.0. After the 4αGTase treatment on amylose, residual linear maltooligosaccharides were removed by β-amylase. CA was analyzed using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectroscopy. DgαGT produced CA with similar yield 53% to the other enzymes but needed less time to start CA production. Degree of polymerization (DP) of CA produced by DgαGT ranged broadly from DP 5 to DP 43. Further analyses need to be conducted for concluding prominent characteristics of DgαGT among bacterial αGTs.
저자
Sa-Rang Kim [ Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea ]
jong-Tae Park [ Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Korea ]
Jung-Eun Kim [ 2Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Korea ]
Jae-Han Kim [ Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.