Earticle

초록

영어

Age-related biochemical and physiological changes have been found in all of the body's cells, tissues, and organs, affecting the function of all body systems. Glycosylation also undergoes changes in the pathophysiological process of aging. Glycosylation, which is highly sensitive to the biochemical environment, is a common post-translational modification of proteins, with over 50% of all human proteins glycosylated. Unlike N-glycans, the research of O-glycan for aging and longevity biomarkers are more challenging owing to the absence of abroad-specificity glycosidase for the release of O-glycans from glycoproteins. In this study, O-glycans in mouse skin tissues were chemically released by reductive β-elimination and analyzed by nano-LC chip/Q-TOF mass spectrometry (Nano-LC/MS). O-glycan enrichment was dramatically improved by optimized lipid removal prior to glycan release. The 20 O-glycan compositions were found in mouse skin tissues by Nano-LC/MS, and O-glycan structures were elucidated by tandem MS. Isomer-specific glycan analysis was successfully performed by accurate mass Q-TOF MS coupled with chip-based liquid chromatography. The alteration of O-glycans during aging was monitored by quantitative analysis. Furthermore, we could identify the relative contribution of neutral, sialylated and fucosylated glycan types. Sialylation was found to be highly correlated with the aging process. Further studies with larger sample sets will help confirm the initial data and lead to more confident detection of aging biomarkers.

저자

• Bum Jin Kim [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]
• Hyoung Jin Jeong [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]
• Serenus Hua [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]
• Sureyya Ozcan [ Department of Biochemistry, University of California Davis, Davis, CA, USA ]
• Lauren Dimapasoc [ Department of Biochemistry, University of California Davis, Davis, CA, USA ]
• Ik-Soon Jang [ Korea Basic Science Institute, 113 Gwahangno, Yuseong-gu, Daejeon, Korea ]
• Jong-Soon Choi [ Graduate School of Analytical Science and Technology, Chungnam National University, Korea Basic Science Institute, 113 Gwahangno, Yuseong-gu, Daejeon, Korea ]
• Hyun Joo An [ Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614