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Enzymatic Modification of 7,8-Dihydroxyflavone by Methylation and Glycosylation

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2013 한국당과학회 동계학술대회 (2013.02)바로가기
  • 페이지
    pp.41-41
  • 저자
    Niranjan Koirala, Ramesh Prasad Pandey, Jae Kyung Sohng
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A212703

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원문정보

초록

영어
Methylation and glycosylation are two different but vital modification processes in plants to generate diversified secondary metabolites. Methylation increases the lipophilicity of compounds whereas glycosylation enhances the solubility. Most of the methylated and prenylated natural compounds are significantly active against cancers. 7, 8-dihydroxyflavone is a member of a flavonoid family of chemicals, which are abundant in fruits and vegetables. The compound’s selective effects suggest that it could be the founder of a new class of brain-protecting drugs as it can cross the blood-brain barrier by triggering tyrosine kinase B (TrkB) proving it could be a powerful anti-oxidant and neuroprotectant drug. In this study, we used E. coli BL21 (DE3) expressing SPOMT 2884, a Streptomyces peucetius ATCC 27952 derived O-methyltransferase, cell as a biocatalyst for the production of methylated derivative of 7, 8-Dihydroxy flavone. The supplementation of 0.2 mM of 7, 8-dihydroxyflavone in the growing induced culture of E. coli BL21 (DE3) harboring pET28-SPOMT2884 recombinant resulted in the production of a mono methylated compound which was confirmed by HPLC (Rt:17 min) and high resolution LC-QTOF-ESI/MS (m/z+ 269.08). Further, this enzymatically synthesized methylated derivative of 7, 8-DHF was purified in large amount and used as a substrate for in-vitro glycosylation by Yjic, a glycosyltransferase from Bacillus licheniformis DSM13. This reaction mixture analysis revealed the presence of glycosylated product which was analyzed by TLC and HPLC. Further structural elucidation is necessary for the confirmation of methylation and glycosylation positions. However, this study reveals a method that might be useful for the enzymatic biosynthesis of the methylated compound and subsequent modification of the same by glycosylation.

저자

  • Niranjan Koirala [ Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University ]
  • Ramesh Prasad Pandey [ Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University ]
  • Jae Kyung Sohng [ Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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