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Structural analysis and bioactivities of a polysaccharide isolated from Capsosiphonfulvescence (Masaengi)

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  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2013 한국당과학회 동계학술대회 (2013.02)바로가기
  • 페이지
    pp.29-29
  • 저자
    Doo Jin Choi, Ji Won Choi, Jisun Lee, Chang Won Lee, Seul Lee, Andriy Synytsya, Yong Il Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A212696

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원문정보

초록

영어
A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean green alga Maesaengi Capsosiphon fulvescens mainly by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The results of present study performed for monosaccharide composition of this polysaccharide using HPLC of PMP labelled sugars, HPAEC-PAD, GC-FID and TLC, clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (44–52 mol %) and rhamnose (34–45 mol %), while mannose and galactose are present at much lesser extent or in negligible amount. These extensive monosaccharide analyses and correlation NMR measurements confirmed that it is a sulphated glucuronorhamnoxylan (ulvan) type polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant invitro anticoagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. Also, Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. The results of this study demonstrated that the SPS-CF isolated from Korean C. fulvescens Maesaengi is an ulvan type polysaccharide and can be considered as potential anticoagulant and immunostimulating agent.

저자

  • Doo Jin Choi [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]
  • Ji Won Choi [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]
  • Jisun Lee [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]
  • Chang Won Lee [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]
  • Seul Lee [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]
  • Andriy Synytsya [ Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology in Prague, Technicka 5, 166 28 Prague 6, Czech Republic ]
  • Yong Il Park [ Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), TheCatholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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