Earticle

초록

영어

In mammal, unfertilized oocytes remain in the oviduct or under in vitro culture, which is called "oocyte aging". This asynchrony negatively affects fertilization in pre- and post-implantation embryo development. Caffeine a phosphodiesterase inhibitor is known to rescue oocyte aging in several species. The objective of this study is to determine the cytoskeleton distribution in aged oocytes and the embryo developmental ability of aged oocytes in the present or absence of caffeine during maturation. Caffeine treatment increased the incidence of normal spindle assembly of aged oocytes (treatment, 67.57±4.11% aging, 44.61±6.4%) and no significant differences compared to control group. Fluorescence values were compared using ROS (Reactive oxidation species) stain. Fluorescence values appear of control group intensity rate (51.53.±3.80), aging group (68.10±5.54) and treatment of caffeine (45.04±2.98). Aged oocytes that were derived from addition of caffeine to the IVM (in vitro maturation) medium had significantly increased 2-cell that developed to the blastocyst stage compared to the aging group. Blastocysts, derived from caffeine treatment group, significantly increased the total cell number compare aging (90.44±10.18 VS 67.88±7.72). Apoptotic fragments of genomic DNA were measured in individual embryo using TUNEL assay. Blastocyst derived from caffeine treatment group decreased significantly the apoptotic index compared to blastocyst derived from aging group. In conclusion, we inferred that the caffeine treatment during oocyte aging can improve the developmental rate and quality in bovine embryos developing in vitro.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Bovine Oocytes and In Vitro Aging of Bovine Oocytes
  In Vitro Fertilization of Bovine Oocytes and Embryo Culture
  During In Vitro Maturation Treatment of Caffeine
  Immunofluorescence and Measurement of ROS
  Confocal Microscopy
  Real Time Reverse Transcription Polymerase Chain Reaction(real time RT-PCR)
  Statistical Analysis
 RESULTS
  Changes of Meiotic Resumption in Bovine Oocytes Aged For 42 h
  Changes of Fertility and Development in Bovine Oocytes Aged for 42 h
  Effect of Meiotic Resumption in Aging Bovine Oocytes with Caffeine Treatment
  Effect of Fertility and Development in Aging Bovine Oocytes with Caffeine Treatment
  Effect of Apoptosis in Bovine Oocytes Aged for 42 h with and without Caffeine Treatment
 DISCUSSION
 REFERENCES

키워드

저자

• Hyun-Yong Choi [ Jeju National University Stem Cell Research Center, Jeju National University, Jeju, 690-756, South Korea ]
• Sung-Hyun Lee [ Department of Animal Sciences, Chungbuk National University, Cheongju, 361-763, South Korea ]
• Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University, Cheongju, 361-763, South Korea ]
• Seung-Eun Lee [ Jeju National University Stem Cell Research Center, Jeju National University, Jeju, 690-756, South Korea ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Cheongju, 361-763, South Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636