Earticle

초록

영어

To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Protein Preparation for 2-DE
  2-DE and Image Analysis
  Protein Identification
 RESULTS AND DISCUSSION
  Proteomic Profiling: Forty Six Proteins Identified (MALDI-ToF-ToF, 19 Proteins; LC-MS-MS, 37 Proteins)
  Abundant Protein Removals 2-DE Proteome Image of Plasma Partially Depleted with Abundant Proteins
 REFERENCES

키워드

저자

• Jin Young Jeong [ Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. ]
• Jin Sun Nam [ Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. ]
• Mi Rim Park [ Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. ]
• Jang Mi Kim [ Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. ]
• Hak Jae Jeong [ Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea ]
• Kyung Woon Kim [ Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea ]
• Hyun-Jeong Lee [ Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea. ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636