Earticle

초록

영어

Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myogenic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and PPARγ increased in rosiglitazone treatment. In study 3, we examined the effect of dexamethasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and PPARγ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblastogenic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHOS
  Animals
  Sample Preparation and Satellite Cell Culture
  Alizarin Red Staining
  Oil Red O Staining
  Masson’s Trichrome Staining
  Real-Time PCR Analysis
  Statistical Analyses
 RESULTS AND DISCUSSION
  Dose-Dependent Effect of Insulin and FBS in Porcine Satellite Cells during Myogenic Differentiation
  Rosiglitazone Regulates Adipogenic Genes in DifferentiatedPorcine Satellite Cells
  Dexamethasone Enhances Differentiatiation in Porcine Satellite Cell
  Determination of a Unique Model during Porcine Satellite Cell
 REFERENCES

키워드

저자

• Jin Young Jeong [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Jang Mi Kim [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Ramanna Valmiki Rajesh [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Sekar Suresh [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Gul Won Jang [ Division of Planning and Coordination, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Kyung-Tai Lee [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Tae Hun Kim [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Mina Park [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Hak Jae Jeong [ Division of Animal Biotechnology, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Kyung Woon Kim [ Biotechnology, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Yong Min Cho [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ]
• Hyun-Jeong Lee [ Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea. ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636