Earticle

초록

영어

The swine is one of the most widespread mammalian throughout the whole world. Presently, many studies concerning microsatellites in swine, especially domestic pigs, have been carried out in order to investigate general diversity patterns among either populations or breeds. Until now, a lot of time and effort spend into a single PCR method. But simple and more rapid multiplex PCR methods have been developed. The purpose of this study is to develop a robust set of microsatellites markers (MS marker) for traceability and individual identification. Using multiplex-PCR method with 23 MS marker divided 2 set, various alleles occurring to 5 swine breed (Berkshire, Landrace, Yorkshire, Duroc and Korea native pig) used markers to determine allele frequency and heterozygosity. MS marker found 4 alleles at SW403, S0227, SWR414, SW1041 and SW1377. The most were found 10 alleles at SW1920. Heterozygosity represented the lowest value of 0.102 at SWR414 and highest value of 0.861 at SW1920. So, it was recognized appropriate allele frequency for individual identification in swine. Using multiplex-PCR method, MS markers used to determine individual identification biomarker and breed-specific marker for faster, more accurate and lower analysis cost. Based on this result, a scientific basis was established to the existing pedigree data by applying genetics additionally. Swine traceability is expected to be very useful system and be conducted nationwide in future.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and Extraction of DNA from Blood
  MS Marker for Allele Analysis
  Multiplex PCR and MS Analysis
  Statistic Analysis
 RESULTS AND DISCUSSION
 REFERENCES

키워드

저자

• Lee-Kung Kim [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Chang-Min Park [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Sun-Ae Park [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Seung-Chang Kim [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Hoyoung Chung [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Han-Ha Chai [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Gyeong-Yong Jeong [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
• Bong-Hwan Choi [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636