Earticle

초록

영어

Sperm capacitation refers to polymerization of filamentous (F)-actin from globular (G)-actin. While the role of actin- related protein 2/3 (Arp2/3) complex in actin polymerization is well appreciated, the underlying mechanism(s) and its relationship with capacitation are poorly understood. Therefore, to evaluate the potential role of Arp2/3 complex on capacitation, bovine spermatozoa were incubated with multiple doses (1, 10 and 100 μM) of CK-636, an inhibitor of Arp2/3 complex with heparin. The cellular localization of the Arp2/3 complex in spermatozoa was identified by immunohistochemistry, whereas western blot was also applied to detect the protein tyrosine phosphorylation of sperm proteins. Additionally, sperm motility and kinematic parameters were evaluated using a computer-assisted sperm analysis system. CK-636 resulted in significant changes in the ratio of Arp2/3 complex localization between acrosome and equatorial region of the spermatozoa. Short-term exposure of spermatozoa to 100 μM of CK-636 significantly decreased sperm motility, however a non-detectable effect on protein tyrosine phosphorylation was observed during capacitation. On the basis of these results, we propose that Arp2/3 complex is associated with morphological changes during capacitation and compromised sperm motility.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Media
  Semen Source and Sperm Preparation
  Immunocytochemistry (ICC)
  Computer-Assisted Sperm Analysis (CASA)
  Western Blot Analysis
  Statistical Analysis
 RESULTS
  Change of Arp2/3 Complex Localization Treated with CK-636
  Effect of CK-636 on Sperm Motility
  Identification of Tyrosine Phosphorylated Proteins
 DISCUSSION
 REFERENCES

키워드

저자

• June-Sub Lee [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Yoo-Jin Park [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Jin Kim [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Md Saidur Rahman [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Woo-Sung Kwon [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Sung-Jae Yoon [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Young-Ah You [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ]
• Myung-Geol Pang [ Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University, Anseong 456-756, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636